Feasibility of Ex Vivo Margin Assessment with Hyperspectral Imaging during Breast-Conserving Surgery: From Imaging Tissue Slices to Imaging Lumpectomy Specimen

Abstract

Developing algorithms for analyzing hyperspectral images as an intraoperative tool for margin assessment during breast-conserving surgery requires a dataset with reliable histopathologic labels. The feasibility of using tissue slices hyperspectral dataset with a high correlation with histopathology for developing an algorithm for analyzing the images from the surface of lumpectomy specimens was investigated. We presented a method to acquire hyperspectral images from the lumpectomy surface with a high correlation with histopathology.