Genomic organization of the mouse beta 1 gene: conservation of the beta 1D but not of the beta 1B and beta 1C integrin splice variants.

Abstract

We have determined the genomic organization of the 3'-region of the murine beta 1 gene and cloned the murine beta 1D integrin splice variant. Overlapping genomic clones encompassing the region of the beta 1D-specific exons were isolated from a phage lambda FIXII library, mapped and partially sequenced. All of the exon-intron junctions identified in the murine beta 1 gene fit with the consensus splice donor and acceptor sequences and occur at the same positions as in their human counterparts. cDNA clones for the beta 1D integrin were isolated from a murine skeletal muscle library. The human and murine beta 1D sequences are conserved at the nucleotide (93%) and amino acid (100%) level, suggesting an important role of this muscle-specific variant throughout mammalian phylogenesis. In contrast, murine sequences for beta 1B are very different from human beta 1B at both the nucleotide as well as amino acid level. Moreover, no specific polyadenylation signal for the beta 1B variant could be identified in genomic clones, suggesting that this variant is not present in the mouse. Finally, we were not able to identify a murine beta 1C splice variant by sequencing analysis, Southern hybridization techniques or polymerase chain reaction of mRNA from platelets. These findings indicate that the beta 1B and beta 1C variants emerged relatively late in the phylogenesis of the beta 1 integrin family.