siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.

Abstract

We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.

More about this publication

Nature methods
  • Volume 13
  • Issue nr. 6
  • Pages 501-4
  • Publication date 01-06-2016

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