Justina Kazokaite

Technician

In April 2019, Justina joined Protein Facility at NKI. Justina is responsible for the expression of recombinant proteins in bacteria, insect cells (using baculovirus) and mammalian cells, followed by purification using automated chromatography procedures. The production size can vary from analytical to larger scale, depending on request and application. Moreover, Justina has acquired experience in a number of biophysical assays, such as ITC, TSA and nanoDSF, and protein crystallisation.

Protein production

Molecular Biology

Biophysics (ITC, TSA and nanoDSF)

Work experience

2004-2008: BSc in Biochemistry at Vilnius University, Lithuania (thesis title: “Production of recombinant human carbonic anhydrase VI, characterization of its stability and the measurements of inhibitor binding”);
2008-2012: MSc in Biochemistry at Vilnius University, Lithuania (thesis title: “Thermodynamic analysis of sulfonamide ligand binding to human carbonic anhydrase VI”);
2015 (2 months): internship at Tampere University, Finland (topic: toxicity studies using zebrafish);
2015 (2 months): internship at Kaiserslautern University, Germany (topic: selective inhibition of carbonic anhydrase IX in Xenopus oocytes);
2016-2017 (8 months): two internships at Maastricht University, The Netherlands (topic: functional effects of lead compounds in cancer cultures);
2014-2018: PhD in Biochemistry at Vilnius University, Lithuania (thesis title: “Investigation of human carbonic anhydrase VI and IX inhibitor efficacy and toxicity in biological systems”).

Justina Kazokaite

Technician

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