Equipment & technologies

Analytical flow cytometers

Aurora Full Spectrum Flow Cytometer (Cytek)

The Aurora is a 5-laser Full Spectrum Flow Cytometer that can be used to explore the wide range of immune cell types that are involved in combatting cancer. Compared to conventional flow cytometers, this instrument allows for deeper probing of cell function and make it easy to investigate cells that show relatively high auto-fluorescence.

BD FACSSymphony A5 SORP (BD Biosciences)

The FACSSymphony A5 is a custom ordered 32 parameter flow cytometer that is used to explore the wide range of immune cell types that are involved in combatting cancer, and their functions.
It allows for simultaneous detection of 30 fluorochromes and with low electronic noise, it can detect very dim signals.

This feature also makes it ideal for the application of the combinatorial coding method to investigate specific CD8 T cell responses to a large number of cancer associated neo-antigens simultaneously.

BD LSR-II SORP (BD Biosciences)

Like the FACSSymphony A5 SORP, the LSR-II SORP is a custom ordered flow cytometer that is equipped with 5 lasers and can detect up to 18 parameters simultaneously.

2 BD LSRFortessas (BD Biosciences)

We have 2 BD LSRFortessa instruments. Both flow cytometers are equipped with 4 lasers, and can detect up to 16 or 18 fluorescent labels simultaneously. These instruments are the work horses of our Facility. Their optical configuration ensures large degree of flexibility in fluorochrome choice.

Attune NxT Acoustic Focusing Cytometer (Thermo Fisher Scientific)

The Attune NxT is a 3-laser system that allows the use of up to fluorochromes simultaneously. The instrument is designed to maintain resolution at high flow rates. This makes the Attune NxT excellently suited for rare event detection. Additionally, the instrument is used for cell cycle analysis and absolute cell counts from whole blood samples.

Intellicyt® iQue Screener PLUS (Sartorius)

This flow cytometer has 3 lasers and can detect up to 13 fluorochromes. The iQue Screener PLUS is excellently suited to perform screens from a large number of samples with small sample volumes. One can think of small molecule or drug discovery assays. The instrument is also excellent for bead based assays.

Cell sorters

The Flow Cytometry Facility harbors a total of 4 cell sorters. Most sorters are located inside biological safety cabinets. This allows us to perform cell sorting under BSL II+ (ML-II+) biosafety conditions.

3 FACSAria ^TM Fusion cell sorters (BD Biosciences)
1 MoFlo Astrios (Beckman Coulter)

3 FACSAria Fusions (BD Biosciences)

The FACSAria Fusions are cuvette-based sorters that are equipped with 3, 4 or 6 lasers, and can detect from 9 to 18 different fluorescent labels simultaneously. The sorters can be set-up to sort cells up to 25-30 micrometer in diameter when in single cell suspension. Four cell population subsets may be collected simultaneously from a single sample when sorting into Eppendorf of 5ml tubes for bulk sorting. Alternatively, single cells or small number of cells can be sorted into plates, from 6-well to 384 well plates.

MoFLo Astrios (Beckman Coulter)

The MoFLo Astrios is a jet-in-air system with 4 lasers and 16 fluorescence detectors. Unlike the other cell sorters, the Astrios is a Jet-in-air system, which allows more flexibility in the regulation of the flow and sorting speed. It's also possible to collect up to 6 cell populations simultaneously from a single sample. Cells can be collected in Eppendorf, 5ml tubes, 15ml and 50ml tubes for bulk sorting. Alternatively, single cells or small number of cells can be sorted into plates, from 6-well to 1,536-well plates.

Equipment & technologies

Analytical flow cytometers

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